International Journal of Medical Discoveries

This study aimed to compare the antimicrobial and versatility of bioceramic sealer with nanoparticles in relation to root canal dentin. Materials and Methods: Totalfill BC sealer, 2.3% and 15% volumes of Silver and chitosan nanoparticles, were added, respectively. Flexibility of root dentin was assessed in terms of thirty single-rooted human teeth, which have been prepared and obturated by the use of the lateral condensation method. The longitudinal sectioning of samples was performed, and the gap area (expressed as a percentage) was indicated at the following magnifications: x45 and x1000, using a stereomicroscope and a scanning electron microscope, respectively. After positioning the sealer under the spectrophotometer, the antimicrobial activity of the sealer was determined against E. faecalis using a direct contact test to assess its bacteriostatic nature. The data indicated a parametric distribution; therefore, intergroup comparisons were performed using one-way ANOVA, followed by the Tukey post hoc test, and intragroup comparisons were performed using ANOVA, followed by the Bonferroni post hoc test. Findings: The adaptability test with SEM results indicated that the highest gap value was obtained in BC + Silver NP (0.59 ± 0.27), followed by BC (0.29 ± 0.15) and then BC + Chitosan NP (0.22 ± 0.16). The gap value between BC and + Silver NP was significantly greater than the gap values between other groups, and this was shown on post hoc pairwise comparisons. According to the direct contact test, the highest value, indicating the highest bacterial growth, was observed in BC + Chitosan NP (0.19 ± 0.01), followed by BC (0.18 ± 0.01), and the lowest value was obtained in BC Silver NP (0.17 ± 0.01). Post hoc pairwise tests revealed a significant difference in abundance between the BC + Chitosan NP group and the other groups (p < 0.001). Conclusion: When incorporated into the bioceramic sealer, nanoparticles can significantly enhance the properties of the latter.